Samaneh mohammadjavad

• Executed PCR, gene cloning, and MLST workflows to identify bacterial isolates, assess genetic variation, and support molecular characterization efforts

• Led advanced molecular workflows—including PCR, gene cloning, and MLST—to characterize bacterial isolates, assess genetic diversity, and support vaccine-related research.
• Optimized Diphtheria vaccine production by performing toxin extraction, detoxification, and high-precision protein purification using chromatography and dialysis systems.
• Applied analytical protein profiling via SDS-PAGE to monitor antigen integrity and validate purification efficiency during vaccine development.
• Executed comprehensive phenotypic assays (hemolytic activity, phospholipase, gelatinase, amylase) and conducted standardized antibiograms to evaluate virulence determinants and antimicrobial resistance.
• Integrated wet-lab and computational approaches by generating heat-maps and phylogenetic trees, enhancing interpretation of genomic and biochemical data.
• Maintained rigorous biosafety practices and produced high-quality documentation ensuring reproducibility, regulatory alignment, and smooth collaboration within multidisciplinary research teams.

• Protein Purification & Analytical Techniques
Performed SDS-PAGE and protein profiling to evaluate antigen purity and confirm efficiency of purification protocols.

• Phenotypic & Antibiotic Resistance Testing
Conducted hemolysis, phospholipase, amylase, gelatinase assays, and comprehensive antibiograms to characterize virulence factors and antimicrobial resistance patterns.