ISABEL PEREZ

I have published several scientific papers:

GOSALBES, MJ., PÉREZ.-ARELLANO, I., ESTEBAN, CD., GALÁN, JL. PÉREZ-MARTÍNEZ, G.

Use of lac regulatory elements for gene expression in Lactobacillus casei.

2001, Le Lait 81:29-35.

PÉREZ-ARELLANO, I, ZUÑIGA, M., PEREZ-MARTINEZ, G. Construction of wide-host range vectors for the cloning of DNA fragments protected by transcriptional terminators.

2001, Plasmid 46, 106-116.

GIL M.TERESA,* PEREZ-ARELLANO, I *, BUESA J, PÉREZ-MARTÍNEZ, G.

* These authors have contributed equally.

Secretion of the vp8* protein in Lactococcus lactis.

2001, FEMS Microbiol Letters. 203, 269-274.

PEREZ-ARELLANO, I, PÉREZ-MARTÍNEZ, G.

Structural features of the lac promoter affecting gusA expression in Lactobacillus casei.

2002, Current Microbiology 45(3), 191-6.

2003, FEMS Microbiol Letters 222 (1), 123-7.

PEREZ-ARELLANO I, GIL-ORTIZ F, CERVERA J, RUBIO V.

Glutamate-5-kinase from Escherichia coli: gene cloning, overexpression, purification and crystallization of the recombinant enzyme and preliminary X-ray studies.

2004, Acta Crystallogr D Biol Crystallogr. 60, 2091-4.

PEREZ-ARELLANO I, RUBIO V, CERVERA J.

Dissection of Escherichia coli glutamate 5-kinase: functional impact of the deletion of the PUA domain.
2005, FEBS Lett. 579(30):6903-8.

AIRES KA, CIANCIARULLO AM, CARNEIRO SM, VILLA LL, BOCCARDO E, PEREZ-MARTINEZ G, PEREZ-ARELLANO I, OLIVEIRA ML, HO PL.

Production of HPV-16 L1 Virus-Like Particles by recombinant Lactobacillus casei.

2006, Appl Environ Microbiol. 72(1):745-52.

PEREZ-ARELLANO I, RUBIO V, CERVERA J.

Mapping active site residues in glutamate-5-kinase. The substrate glutamate and the feed-back inhibitor proline bind at overlapping sites.

2006, FEBS Letters, 580, 6247-6253.

MARCO-MARÍN C, GIL-ORTIZ F, PEREZ-ARELLANO I, CERVERA J, FITA I AND RUBIO V.

A novel two domain architecture within the amino acid kinase family revealed by the crystal structure of Escherichia coli glutamate 5-kinase.

2007, J. Mol. Biol. 367, 1431–1446

PEREZ-ARELLANO I, GALLEGO J, CERVERA J.

The PUA domain - a structural and functional overview.

2007 FEBS J. 274, 4972-84.

PÉREZ-ARELLANO I, GOZALBO-ROVIRA R, MARTÍNEZ AI, CERVERA J.

Expression and purification of recombinant human MCT-1 oncogene in insect cells.

Protein J. 2010 Feb;29(2):69-74.

PÉREZ-ARELLANO I, CARMONA-ALVAREZ F, MARTÍNEZ AI, RODRÍGUEZ-DÍAZ J, CERVERA J.

Pyrroline-5-carboxylate synthase and proline biosynthesis: from osmotolerance to rare metabolic disease.

Protein Sci. 2010 Mar;19(3):372-82. Review.

PÉREZ-ARELLANO I, CERVERA J.

Glutamate kinase from Thermotoga maritima: characterization of a thermophilic enzyme for proline biosynthesis.

Extremophiles. 2010 Jul;14(4):409-15.

MARTÍNEZ AI, PÉREZ-ARELLANO I, PEKKALA S, BARCELONA B, CERVERA J.

Genetic, structural and biochemical basis of carbamoyl phosphate synthetase 1 deficiency.

Mol Genet Metab. 2010 Dec;101(4):311-23.

PÉREZ-ARELLANO I, CARMONA-ALVAREZ F, GALLEGO J, CERVERA, J.

Molecular mechanisms modulating glutamate kinase activity. Identification of the proline feedback inhibitor binding site. J Mol Biol. 2010, 404(5):890-901.

DÍEZ C, MARTÍNEZ AI, PEKKALA S, BARCELONA B, PÉREZ-ARELLANO I, SUMMAR M, RUBIO V, CERVERA J.

Molecular characterization of carbamoyl phosphate synthetase (CPSI) deficiency using human recombinant CPSI as a key tool.

Human Mutation, 2013 May 3. doi: 10.1002/humu.22349.

PÉREZ-ARELLANO I, SPINOLA-NAMIBIA M, BRAVO J.

Human Drg1 is a potassium dependent Gtpase activated by Lerepo4.

FEBS J. 2013 May 25. doi: 10.1111/febs.12356.

I have published a chapter of a scientific book:

MONEDERO V, PÉREZ-ARELLANO I, VIANA R, ZÚÑIGA M, BUESA J, MONTAVA R, RODRÍGUEZ-DÍAZ J, LEE HO P, SARNO DE OLIVEIRA L, Y PÉREZ-MARTÍNEZ G.

Diseño de probióticos como vectores de vacunas orales y otras moléculas terapéuticas. En: Microorganismos y salud. Bacterias lácticas y bifidobacterias probióticas. Book chapter 14, pág. 172-183. (Juan Miguel Rodríguez Gómez, Ed), Editorial Complutense, Madrid. (2007)

I am part of a patent:

GOSALBES SOLER MJ, PÉREZ ARELLANO I, ESTEBAN NIETO CD, GALÁN JL Y PÉREZ MARTÍNEZ G.

TITULO:

Uso de la secuencia de nucleótidos del promotor de la lactosa de lactobacillus casei para regular la expresión génica a través de una proteína antiterminadora.

Nº registro 2000-00897

PRIORITY COUNTRY SPAIN PRIORITY DATE 7 de Abril de 2000

ENTITY CSIC

OTHER COUNTRIES Europe

I have solved the 3D structure of a mutant enzyme of glutamate kinase without the PUA domain of Escherichia coli. PDB id: 2W21

-REDACTORA MÉDICA DEL ARTÍCULO:

As a medical writer I wrote the following paper: Maintenance therapy with vinflunine plus best supportive care versus best supportive care alone in patients with advanced urothelial carcinoma with a response after first-line chemotherapy (MAJA; SOGUG 2011/02): a multicentre, randomised, controlled, open-label, phase 2 trial. García-Donas J, Font A, Pérez-Valderrama B, Virizuela JA, Climent MÁ, Hernando-Polo S, Arranz JÁ, Del Mar Llorente M, Lainez N, Villa-Guzmán JC, Mellado B, Del Alba AG, Castellano D, Gallardo E, Anido U, Del Muro XG, Domènech M, Puente J, Morales-Barrera R, Pérez-Gracia JL, Bellmunt J. LANCET ONCOLOGY, 2017 May;18(5):672-681.

• GIL M.TERESA,
• PEREZ-ARELLANO, I
• BUESA J, PÉREZ-MARTÍNEZ, G
• These authors have contributed equally
• Secretion of the vp8
• Protein in Lactococcus lactis
• 2001, FEMS Microbiol Letters
• 203, 269-274
• PEREZ-ARELLANO, I, PÉREZ-MARTÍNEZ, G
• Structural features of the lac promoter affecting gusA expression in Lactobacillus casei
• 2002, Current Microbiology 45(3), 191-6.
• PEREZ-ARELLANO, I, PÉREZ-MARTÍNEZ, G
• Optimisation of the green fluorescent protein (GFP) expression from a lactose-inducible promoter in Lactobacillus casei
• 2003, FEMS Microbiol Letters 222 (1), 123-7
• PEREZ-ARELLANO I, GIL-ORTIZ F, CERVERA J, RUBIO V
• Glutamate-5-kinase from Escherichia coli: gene cloning, overexpression, purification and crystallization of the recombinant enzyme and preliminary X-ray studies
• 2004, Acta Crystallogr D Biol Crystallogr
• 60, 2091-4
• PEREZ-ARELLANO I, RUBIO V, CERVERA J
• Dissection of Escherichia coli glutamate 5-kinase: functional impact of the deletion of the PUA domain
• 2005, FEBS Lett
• 579(30):6903-8
• AIRES KA, CIANCIARULLO AM, CARNEIRO SM, VILLA LL, BOCCARDO E, PEREZ-MARTINEZ G, PEREZ-ARELLANO I, OLIVEIRA ML, HO PL
• Production of HPV-16 L1 Virus-Like Particles by recombinant Lactobacillus casei
• 2006, Appl Environ Microbiol
• 72(1):745-52
• PEREZ-ARELLANO I, RUBIO V, CERVERA J
• Mapping active site residues in glutamate-5-kinase
• The substrate glutamate and the feed-back inhibitor proline bind at overlapping sites
• 2006, FEBS Letters, 580, 6247-6253
• MARCO-MARÍN C, GIL-ORTIZ F, PEREZ-ARELLANO I, CERVERA J, FITA I AND RUBIO V
• A novel two domain architecture within the amino acid kinase family revealed by the crystal structure of Escherichia coli glutamate 5-kinase
• 2007, J
• Mol
• Biol
• 367, 1431–1446
• PEREZ-ARELLANO I, GALLEGO J, CERVERA J
• The PUA domain - a structural and functional overview
• 2007 FEBS J
• 274, 4972-84
• PÉREZ-ARELLANO I, GOZALBO-ROVIRA R, MARTÍNEZ AI, CERVERA J
• Expression and purification of recombinant human MCT-1 oncogene in insect cells
• Protein J
• 2010 Feb;29(2):69-74
• PÉREZ-ARELLANO I, CARMONA-ALVAREZ F, MARTÍNEZ AI, RODRÍGUEZ-DÍAZ J, CERVERA J
• Pyrroline-5-carboxylate synthase and proline biosynthesis: from osmotolerance to rare metabolic disease
• Protein Sci
• 2010 Mar;19(3):372-82
• Review
• PÉREZ-ARELLANO I, CERVERA J
• Glutamate kinase from Thermotoga maritima: characterization of a thermophilic enzyme for proline biosynthesis
• Extremophiles
• 2010 Jul;14(4):409-15
• MARTÍNEZ AI, PÉREZ-ARELLANO I, PEKKALA S, BARCELONA B, CERVERA J
• Genetic, structural and biochemical basis of carbamoyl phosphate synthetase 1 deficiency
• Mol Genet Metab
• 2010 Dec;101(4):311-23
• PÉREZ-ARELLANO I, CARMONA-ALVAREZ F, GALLEGO J, CERVERA, J
• Molecular mechanisms modulating glutamate kinase activity
• Identification of the proline feedback inhibitor binding site
• J Mol Biol
• 2010, 404(5):890-901
• DÍEZ C, MARTÍNEZ AI, PEKKALA S, BARCELONA B, PÉREZ-ARELLANO I, SUMMAR M, RUBIO V, CERVERA J
• Molecular characterization of carbamoyl phosphate synthetase (CPSI) deficiency using human recombinant CPSI as a key tool
• Human Mutation, 2013 May 3
• Doi: 10.1002/humu.22349
• PÉREZ-ARELLANO I, SPINOLA-NAMIBIA M, BRAVO J
• Human Drg1 is a potassium dependent Gtpase activated by Lerepo4
• FEBS J
• 2013 May 25
• Doi: 10.1111/febs.12356
• CAPÍTULOS DE LIBRO
• MONEDERO V, PÉREZ-ARELLANO I, VIANA R, ZÚÑIGA M, BUESA J, MONTAVA R, RODRÍGUEZ-DÍAZ J, LEE HO P, SARNO DE OLIVEIRA L, Y PÉREZ-MARTÍNEZ G
• Diseño de probióticos como vectores de vacunas orales y otras moléculas terapéuticas
• En: Microorganismos y salud
• Bacterias lácticas y bifidobacterias probióticas
• Book chapter 14, pág
• 172-183
• (Juan Miguel Rodríguez Gómez, Ed), Editorial Complutense, Madrid
• (2007)
• PATENTES
• GOSALBES SOLER MJ, PÉREZ ARELLANO I, ESTEBAN NIETO CD, GALÁN JL Y PÉREZ MARTÍNEZ G
• TITULO:
• Uso de la secuencia de nucleótidos del promotor de la lactosa de lactobacillus casei para regular la expresión génica a través de una proteína antiterminadora
• Nº registro 2000-00897
• PRIORITY COUNTRY SPAIN PRIORITY DATE 7 de Abril de 2000
• ENTITY CSIC
• OTHER COUNTRIES Europe
• OTROS RESULTADOS DESTACADOS: - RESOLUCIÓN DE LA ESTRUCTURA 3D DEL MUTANTE TRUNCADO DE LA ENZIMA GLUTAMATO QUINASA DE Escherichia coli, SIN EL DOMINIO PUA, FORMA apo
• PDB id: 2W21 -REDACTORA MÉDICA DEL ARTÍCULO:
• Maintenance therapy with vinflunine plus best supportive care versus best supportive care alone in patients with advanced urothelial carcinoma with a response after first-line chemotherapy (MAJA; SOGUG 2011, PARTICIPACIÓN EN SIMPOSIOS, CONGRESOS Y REUNIONES CIENTÍFICAS
• PONENCIAS ORALES
• NACIONAL
• DATOS DE VIGILANCIA AMBIENTAL PROCEDENTES DE LAS INSPECCIONES PARA EL CONTROL DE LEGIONELOSIS, EN LA CIUDAD DE VALENCIA DURANTE EL 2018
• Perez-Arellano Isabel, Quero-de Lera Fermín
• Comunicación corta
• XV Congreso de Salud Ambiental,
• Valencia, España, 22-24 mayo 2019
• NACIONAL
• GLUTAMATO-5-QUINASA: ENZIMA CLAVE EN LA REGULACIÓN DE LA BIOSÍNTESIS DE PROLINA
• Pérez-Arellano, I, Gil-Ortiz, F, Rubio V y Cervera, J
• Presentación Oral
• Congreso de la Sociedad Española de Bioquímica y Biología Molecular (SEBBM) Lérida, SEPTEMBER 2004
• COMUNICACIONES EN FORMATO POSTER
• NACIONAL
• RECOMBINANT HUMAN CARBAMYL-PHOSPHATE SYNTHETASE 1 (CPS1): AT LAST A WORKBENCH FOR UNDERSTANDING THE UREA CYCLE DISEASE CPS1 DEFICIENCY
• Diez-Fernandez, C; Martinez, AI; Pekkala, S; Barcelona, B; Perez-Arellano, I; Summar, M; Rubio, V; Cervera, J, THE CRYSTAL STRUCTURE OF ESCHERICHIA COLI GLUTAMATE 5 KINASE (G5K) REVEALS A NOVEL HYBRID AMINO ACID KINASE /PUA DOMAIN TETRAMERIC ARCHITECTURE PERMITTING CATALYSIS, REGULATION, SUBSTRATE CHANELIG AND POSSIBLY GENE REGULATION, NACIONAL
• ESTRUCTURA TRIDIMENSIONAL DE UN MIEMBRO INESPERADO DE LA FAMILIA DE PROTEÍNAS QUE PORTAN EL DOMINIO PUA: LA GLUTAMATO 5-QUINASA (G5K) DE Escherichia coli
• Clara Marco-Marín, Isabel Perez-Arellano, Fernando Gil-Ortiz, Ignacio Fita, Javier Cervera y Vicente Rubio
• Cartel
• Reunión de la Red Nacional de Plegamiento, Estabilidad e Ingeniería de Proteínas
• Bilbao, 9-11 OCTOBER 2006
• INTERNACIONAL
• TOWARDS STRUCTURAL CHARACTERIZATION OF GLUTAMATE 5-KINASE OF ESCHERICHIA COLI
• I
• Perez-Arellano, F Gil-Ortiz, V
• Rubio, J
• Cervera
• Panel y comunicación oral breve
• The 19th International Conference on Arginine and Pyrimidines (ICAP 2004)
• Marburg, Alemania SEPTEMBER 2004
• NACIONAL ¿COMPARTEN LOS ENZIMAS N-ACETIL-L-GLUTAMATO QUINASA, ASPARTOQUINASA Y GLUTAMATO-5-KINASA UNA MISMA ESTRUCTURA BÁSICA Y, Ponencia oral 10 ème Colloque du Club des Bacteries Lactiques
• Strasbourg, Francia
• 2000
• INTERNACIONAL
• EXPRESSION OF RECOMBINANT VP8 PROTEIN OF SIMIAN ROTAVIRUS IN ESCHERICHIA COLI AND IN LACTIC ACID BACTERIA
• Buesa, J., De Souza, C.O., Gil, M.T., Pérez-Arellano, I., Pérez-Martínez, G
• Panel
• CONGRESO XI International Congress on Virology
• Sidney, Autralia
• 9-13 AUGUST, 1999
• INTERNACIONAL
• CONSTRUCTION OF WIDE HOST RANGE SHUTTLE CLONING VECTORS WITH A LACZ CASSETTE PROTECTED BY TRANSCRIPTIONAL TERMINATORS
• Pérez-Arellano, I., Y Pérez-Martínez, G
• Cartel 6th Symposium on LAB: Genetics, metabolism and applications
• Veldhoven, Holanda 16 al 23 SEPTEMBER, 1999 8